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Monday, April 20, 2020 | History

2 edition of Isolation, cloning and tissue distribution of a 500 kDa protein with Ca²⁺ sensing properties found in the catalog.

Isolation, cloning and tissue distribution of a 500 kDa protein with Ca²⁺ sensing properties

Stefan Lundgren

Isolation, cloning and tissue distribution of a 500 kDa protein with Ca²⁺ sensing properties

  • 329 Want to read
  • 15 Currently reading

Published by Swedish University of Agricultural Sciences, Genetic Center, Dept. of Cell Research in Uppsala .
Written in English

    Subjects:
  • Glycoproteins.,
  • Protein engineering.,
  • Molecular cloning.

  • Edition Notes

    Statementby Stefan Lundgren.
    ContributionsSveriges lantbruksuniversitet.
    The Physical Object
    Pagination1 v. (various pagings) :
    ID Numbers
    Open LibraryOL18077985M
    ISBN 109157649537

    Disrupted pancreatic β-cell function is a key event in the pathogenesis of diabetes mellitus, a metabolic disorder resulting in elevated blood sugar levels. β-cells are responsible for the secretion of insulin, which promotes the uptake of blood glucose into peripheral tissue. Additionally, autocrine insulin signalling contributes to the maintenance of properly functioning β-cells. Upon Author: Tobias Albrecht. Other psychometric properties could not be demonstrated sufficiently solid. CONCLUSION: Many psychometric properties of the Community Integration Questionnaire are still poorly evaluated for adults living with a disability other than a traumatic brain injury. However, promising data have been documented in each population included in this review. Isolation of a bacterial enzyme releasing axillary malodor and its use as a screening target for novel deodorant formulations.. PubMed. Natsch, A; Gfeller, H; Gygax, P; Schmid, J. Axillary odor is known since 50 years to be formed upon the action of Corynebacteria on odorless axilla secretions, but the nature of the bacterial enzymes involved in this process remained a mystery.


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Isolation, cloning and tissue distribution of a 500 kDa protein with Ca²⁺ sensing properties by Stefan Lundgren Download PDF EPUB FB2

The deduced protein, amino acids long, is rich in glutamic and aspartic acid residues, endowing p23k with a net negative charge at neutral pH. The protein lacks a signal sequence as well as any transmembrane domains.

Based on predictions of secondary structure, p23k is a globular protein composed of 30% α-helices and 18% β-pleated by: Tissue specific distribution of calcyclin On two-dimensional blots, derived from low ionic strength extracts of EAT cells, polyclonal an- cloning and tissue distribution of a 500 kDa protein with Ca²⁺ sensing properties book raised cells calcyclin reacted only with one protein spot.

The spot was cor- responding to the monomer of calcyclin (fig.1). The purified protein has been characterized with respect to its physical, chemical, and calcium-binding properties.

It has an apparent molecular weight of 63, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis by sedimentation equilibrium centrifugation under nondenaturing by:   To understand the roles of plant vacuoles, we have purified and characterized a major soluble protein from vacuoles of radish (Raphanus sativus cv Tokinashi-daikon) taproots.

The results showed that it is a novel radish vacuole Ca2+-binding protein (RVCaB). RVCaB was released from the vacuolar membrane fraction by sonication, and purified by ion exchange and gel filtration column.

Isolation and characterization of a new Ca 2+ /calmodulin-dependent protein kinase from isoproterenol-stimulated proliferating rat parotid acinar cells January Biochemistry and Cell Biology. A novel kDa protein Isolation (p67 cdpk) was identified in the microsomal membrane fraction of apple (Malus domestica Borkh.

Braeburn) suspension cultures and subsequently found to be active in sink tissues. Microsomal proteins were blotted onto Nylon or polyvinylidenedifluoride membranes, and p67 cdpk assayed by in situ-labelling renatured proteins with [γP]ATP; thin-layer Cited by:   It is the uppermost layer of the Isolation epidermis, thestratum corneum, that provides the vital barrier between the organism and its environment.

The stratum corneum is generated by keratinocytes which migrate from the basal layer to the surface of the epidermis undergoing terminal differentiation, a process which is known to be calcium dependent ().

A 'read' is Isolation each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text.

A novel member of the S protein family, present in human placenta, has been characterized by protein sequencing, cDNA cloning, and analysis of Ca(2+)-binding properties. Since the placenta protein of 95 amino acid residues shares about 50% sequence identity Isolation the brain S proteins alpha and beta, we proposed the name by: Protein Biotechnology: Isolation, Characterization, and Stabilization (Biological Methods) - Kindle edition by Franks, Felix.

Download it once and read it on your Kindle device, PC, phones or tablets. Use features like bookmarks, note taking and highlighting while reading Protein Biotechnology: Isolation, Characterization, and Stabilization (Biological Methods).Manufacturer: Humana.

The term "antibody production" has both general and specific meanings. In the broad sense, it refers to the entire process of creating a usable specific antibody, including steps of immunogen preparation, immunization, hybridoma creation, collection, screening, isotyping, purification, and labeling for direct use in a particular method.

SA1 Ca2+-Binding Protein Isolation the Human Heart Three different blocks of tissue were used. From each block 3 different grids with sections were prepared. Sixteen micrographs were taken from each grid and printed at total magnification of 17 ×. Areas (µm2) of immunolabeled structural components (Ac) of myofibrils were estimated by the.

dalton glycoprotein and dalton peptide inducing selective Ca2+-transport through bilayer lipid membranes were isolated from beef heart homogenate and mitochondria. Micromolar concentrations of these substances were found to increase the conductivity of membranes by 3–4 orders.

Transmembrane Ca2+ gradient induces an electric potential difference whose magnitude Cited by: The Calsequestrin 1 Recombinant Protein Antigen from Novus Biologicals is derived from E. coli. The Calsequestrin 1 Recombinant Protein Antigen has been validated for the following applications: Antibody : ml.

This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.

Through brands you trust — Gibco® cell culture media and feeds, POROS® chromatography resins, and Applied Biosystems® SEQ rapid contaminant tests — Life Technologies is helping to set new manufacturing standards in product quality, safety and fast-to-market efficiency for therapeutic protein production.

View all Products. kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

database using the human TRP3 protein sequence23; This lead to the identification of human short TRP7 (hTRP7). TaqMan real time quantitative RT-PCR was used to investigate the mRNA distribution of hTRP7 in CNS and peripheral tissues, and in Cited by: 0 by The American Society for Biochemistry and Molecular Biology, Inc.

THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. No. 15, Issue of pp. Printed in U. S.A. Glycosylation of Human Protein C Affects Its Secretion, Processing, Functional Activities, and Activation by Thrombin* (Received for publication, Decem).

Distal convoluted tubule cells of the kidney play a crucial role in the determination of the final urinary magnesium excretion. In addition to responding to changesin local divalent cation concentrations, magnesium absorption in these cells is also under the influence from a number of hormones.

Immortalized mouse distal convoluted tubule cells (MDCT) retain a number of the properties ofin vivo. Highly purified recombinant Cas9 protein is designed to combine with in vitro transcribed guide RNA for more efficient and precise genome editing in cells while reducing off-target effects and avoiding unwanted integration of plasmid DNA in the host genome.

CB1 from apple was the first CBK isolated from plant tissue, but this was soon followed by the isolation of MCK from ma 13, OsCBK from rice, AtCBK1 from Arabidopsis and chimeric Ca 2+ –CaM-dependent protein kinases (CCaMKs) from lily and toba All of these CBKs contain an N-terminal domain of variable length and sequence, a protein kinase catalytic domain, a CaM-binding Cited by: The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S Calcium Binding Protein B (SB) and their serial dilutions.

The results were demonstrated by the percentage of calculated concentration to the expected. High serum lipopolysaccharide binding protein is associated with increased mortality in patients with decompensated cirrhosis pubmed Journal of Functional Foods Modulation of gut microbiota and gut-generated metabolites by bitter melon results in improvement in the metabolic status in high fat diet-induced obese rats / Neuropharmacology Vol.

32, No. I, pp./93 $ + Printed in Great Britain Pergamon Press Ltd POSITRON EMISSION TOMOGRAPHIC STUDIES OF [I1C]MDLA POTENTIAL 5-HT3 RECEPTOR RADIOLIGAND: DISTRIBUTION, KINETICS AND BINDING IN THE BRAIN OF THE BABOON R.

CAMSONNE,2'5 LOUISA BARRE,1'2'3 MARIE-CHRISTINE PETIT Cited by: Water-soluble models of heme-protein active sites are obtained via the self-assembly of cationic porphyrins 1 and tetrasulfonato calix[4]arene 2 (K12 = M-1).

Selective binding of ligands either outside or inside the cavity of assemblies 12 via coordination to the zinc center has been observed.

Small ligands such as 4-methylpyridine and 1-methylimidazole are encapsulated, while the. The protein encoded by this gene is a member of the S family of proteins containing 2 EF-hand calcium-binding motifs.

S proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S genes include at least 13 members which are located as a cluster on.

Recombinant Human Protein APCDD1(APCDD1),partial (Yeast-CSB-YPHU -CSB-EPHU Baculovirus-CSB-BPHU Mammalian cell-CSB-MPHU In Vivo Biotinylation in -CSB-EPHU-B)Uniprot No.: Q8J Eag1 is neuron‐specific K+ channel abundantly expressed in the brain and retina.

Subcellular localization and physiological analyses in neurons reveal that Eag1 may participate in Ca2+‐signaling processes Cited by: 2. Pierce Chemical Technical Library Coomassie® Dye Binding Method Another type of protein assay uses protein-binding dyes.

The most popular is based on Coomassie® Brilliant Blue G and is known as the Bradford Method The Coomassie ® Protein Assay is based on the im-mediate absorbance shift from nm to nmFile Size: 15KB.

Plasma membrane‐associated Ca 2+ ‐binding protein–2 (PCaP2) of Arabidopsis thaliana is a novel‐type protein that binds to the Ca 2+ /calmodulin complex and phosphatidylinositol phosphates (PtdInsPs) as well as free Ca 2+.Although the PCaP2 gene is predominantly expressed in root hair cells, it remains unknown how PCaP2 functions in root hair cells via binding to ligands.

High-throughput immune repertoire sequencing has emerged as a critical step in the understanding of adaptive responses following infection or vaccination or in autoimmunity. However, determination of native antibody variable heavy-light pairs (VH-VL pairs) remains a major challenge, and no technologies exist to adequately interrogate the >1 × 10(6) B cells in typical specimens.

In critically ill neonates aged over 48 h and children lipopolysaccharide binding protein is a better marker than procalcitonin, interleukin-6 and C-reactive protein. PMID: Dual role of LBP and CD14 in initiation of proinflammatory signaling and clearance or neutralization of t No.: P (d= µm) on the CD Peptides are desalted and purified during elution with organic solvent containing the MALDI matrix Protein digests to CD microlaboratory Single microstructure Co-crystallized peptides And matrix on the MALDI target in the CD Mass spectrum of BSA showing higher peak intensity and a greater number of peptides.

form moisture detection on lyophilized protein products. Lyophilization is a common formulation for protein pharmaceuticals, and moisture content in final product is typically part of certificate of analysis testing for lyophilized products.

Current chemical titration methods for moisture detection, such as Karl Fischer, require that aFile Size: KB. Illustration about Human antibody (immunoglobulin)protein molecule, chemical structure. Illustration of diagnosis, immunoglobulin, acid - By using a protein-design algorithm that quantitatively considers side-chain packing, the effect of specific steric constraints on protein design was assessed in the core of the streptococcal protein G beta 1 domain.

The strength of packing constraints used in the design was varied, resulting in core sequences that reflected differing amounts of packing specificity.

Fluidigm Corporation announced today a collaboration with BD Biosciences, a segment of BD, to co-host a public seminar series on the isolation and analysis of single cells.

Entitled A Powerful. Protein Amplification and Simple Purification The Recombinant Protein Handbook Antibody Purification Handbook Edition AC ˛ ˆ ˘ ˚ ˘ ˜. "# $ ˜. %ˇ& ˇ %’(% & ’ $ $ ˘ ˚)*˘. + ˜. ("˘ ˆ $ ˛ ˆ ˙ + ˆ $ ˘ Protein Purification Handbook Edition AC, ˘ ˘ ˜ˇˇ ˜ ˇ $ ˘ ˇˇ & ˘ ˇˇ-$.

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[13].Cell surface expression of kDa glucose-regulated protein (GRP78) mediates diabetic nephropathy. JOURNAL OF BIOLOGICAL CHEMISTRY, (19), DOI WoS 1.